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A SIMPLE HPLC METHOD FOR THE ESTIMATION OF ANDROGRAPHOLIDE

Yedugani Lingam, Gajanan Patange, Atish Jayaprakas Salunke


A simple, rapid, selective and quantitative HPLC method has been developed for the determination of Andrographolide. Mobile phase was Water: Acetonitrile (60: 40), Flow rate 1.0 ml/min. Retention time of pure Andrographolide was 5.038 minutes. The content of andrographolide in crude (BNPL/AD/003) was 30% and reasonable pure sample (BNPL/AD/006) purity is 82% respectively. The developed method was found to be simple, robust, rugged, and economic for routine use in the herbal drug industry.


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  • Infraestrutura Nacional de Conhecimento da China (CNKI)
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  • Cosmos SE
  • Biblioteca de Periódicos Eletrônicos
  • Diretório de indexação de periódicos de pesquisa (DRJI)
  • Laboratórios secretos de mecanismos de pesquisa
  • ICMJE

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