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A Validated RP-HPLC Method For Simultaneous Estimation Of Losartan Potassium And Amlodipine In Pharmaceutical Formulation

Aravindraju, S.Rajan, S.Muralidharan, S.N.Meyyanathan, B.Suresh

A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the simultaneous estimation of losartan potassium and amlodipine besylate from pharmaceutical formulation. The method was carried out on a Princeton SPHER C18 (15 cm x 4.6 mm i.d., 5 ) column with a mobile phase consisting of acetonitrile: dipotassiumorthophosphate (adjusted to pH 7.5 using orthophosphoric acid) (50:50 v/v) at a flow rate of 0.7 ml/min. Detection was carried out at 239 nm. Diazepam was used as an internal standard. The retention time of losartan, amlodipine and diazepam was 5.36, 3.76 and 15.47 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can be used for the estimation of these drugs in combined dosage forms.