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Antimicrobial and Anthelmintic efficacy of methanol extract of Putranjiva roxburghii Wall seed

A.Chinmaya, S.J.Sudharshan, N.C.Valleesha, M.L.Sujatha, Namitha C.Yadav, T.R.Prashith Kekuda, A.N.Rajeshwara

Putranjiva roxburghiiWall., commonly called Putranjiva, is a deciduous, evergreen tree and is used in cold, fever, rheumatism and inflammation. The study carried by us describes the antibacterial and anthelmintic potential of methanolic extract of fruits of P. roxburghii in vitro. The dried and powdered Seed material of P. roxburghii was subjected to soxhlet extraction using methanol solvent. The phytochemical analysis was carried. The antibacterial activity was checked using Agar well diffusion method and Anthelmintic assay was conducted in earthworm model. Preliminary phytochemical investigation reveled the presence of saponin, steroid, alkaloids and flavonoids in the extract. A marked antibacterial activity againstGrampositive and Gramnegative bacteria, known to cause food poisoning, was observed. Grampositive bacteria weremore inhibited than Gram negative bacteria. Among fungi tested, A. flavus was found to be more susceptible followed by A. niger and A. nidulans. The extract was found to cause paralysis and death of worms in a relatively short period of time. The results reveals that methanol extract possess active principles that possess antimicrobial and anthelmintic activity. Purification of active constituents and in vivo experiments in animal models are needed to support the ethnomedicinal use of the plant.