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Cost-effective production of pectinase by parametric optimization of Pseudozyma sp. SPJ under submerged fermentation

Sampriya Sharma, Rishi Pal Mandhan, Jitender Sharma

Pseudozyma sp. SPJ was isolated for the production of pectinase and various culture conditions were standardized to have cost effective production. Maximumpectinase titer was achieved at 35ï‚°C for 22 h of incubation. Aproduction broth containing 0.8%citrus peel, 0.125%yeast extract, 0.125%peptone and 10mMCaCl2 (pH8.0)was found to stimulate excellent (653.13 ± 19.32 U ml-1) enzyme production. Optimum inoculum size was found to be 2%when itwas 14 h old. Xylose and lactose were inhibited the enzyme production. Heavy metal ions such as Hg2+, Cu2+, Pb+, Fe2+ and Co2+ adversely affected the pectinase production. The enzyme production was successfully scaled up at laboratory level. An appreciable amount of pectinase was produced by a newly isolated yeast strain with a cost effective mediumunder sub-merged fermentation.