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Degradation and quantification of polyaromatic hydrocarbon by Pseudomonas sp.

T.Kuberan, R.Vidhya Pallavi, J.Anburaj, C.Sundaravadivelan, P.Kumar, V.Prabakaran

Polyaromatic hydrocarbon compounds are hazardous and found to be highly mutagenic and carcinogenic. Biodegradation is an eminent technique for reducing the toxic nature of polyaromatic hydrocarbon in petroleumamended soil by sustainable manner. Pseudomonas sp., having biodegradation propertywas isolated by selectivemedia and characterized by various biochemical test. Pseudomonas aeruginosa and Pseudomonas fluorescence were isolated fromthe petroleumcontaminated area and Pseudomonas stutzeri 2643 obtained fromtheMTCC which is used for the biodegration of PAH. These three cultures were screened for potential PAH degrading bacteria. The poly aromatic hydrocarbon degradation by Pseudomonas aeruginosa, Pseudomonas fluorescence and Pseudomonas stutzeri 2643 obtained were compared with the amount of PAH present in the control (Soil and Petrol) and was estimated by HPLC, which was found to be 55.6% (500µl petrol). The result indicates that the PAH quantity in Pseudomonas aeruginosa treated sample was found to be 23.40% (500µl petrol), in Pseudomonas flourescens treated sample the PAH Quantitywas found to be 12.28%(500µl petrol) and in Pseudomonas Stutzeri 2643 treated sample which revealed 13.91% (500µl petrol). From this study it indicates that Pseudomonas sp., reduces the PAH pollutants from the environment