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Derivative Spectrophotometric Method For Simultaneous Estimation Of Nimesulide And Serratiopeptidase From Tablet Dosage Form

S.J.Daharwal, Saraf Swarnlata, S.Saraf

The first derivative spectrophotometric method for simultaneous estimation of nimesulide(NL) and serratiopeptidase(SP) in two component solid dosage forms has been developed. The method utilizes phosphate buffer pH 7.4 as a solvent. In this solvent system, the first derivative spectrum of NL shows the equal absorbance at 223nm and 250 nm and for the estimation of SP, the difference between the absorbance at 223nm and 250nm was used. The spectrum of SP shows the zero absorbance at 360.5nm, this zero crossing point was used for the estimation of NL. The linearity ranges for NL and SP were 10-90 μg/ml and 2-90 μg/ml, respectively.