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Determination Of Bergenin In Caesalpinia Digyna Rottler By RP-HPLC Method

M.J.N.Chandrasekar, R.Srinivasan, R.Gopinath, M.J.Nanjan, B.Suresh

A simple, selective, rapid and precise reverse phase HPLC method has been developed for the standardization of Caesalpinia digyna root (Family: Leguminosae) using Bergenin as an analytical marker. The method was carried out on a Phenomenex Gemini C18 (25 cm x 4.6 mm i.d.,5 μ) column with a mobile phase consisting of Acetonitrile: Water (30:70 v/v) at a flow rate of 0.8 ml/min. Detection was carried out at 275 nm. Retention time of Bergenin was found to be 4.38.The calibration curve was linear in the range of 0.250 μg/ml to 2.5μg/ml of Bergenin and the correlation coefficient was 0.9999, indicating good linear dependence of peak area on concentration. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantitation. The proposed method can be used for the standardization of Bergenin in Caesalpinia digyna root extract.