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Effect of pH and Incubation Time Against Endoglucanase Activity from Trichoderma reesei and Aspergillus niger with Rice Strawas Substrat

Bambang Dwi Argo, Bambang Rahadi JW and Poppy Diana Sari

Endoglucanase is one important part of the enzyme cellulase, which works to solve bonding polymer â-1,4 which is a part of the amorphous cellulose, and produce cello-oligosaccharides.Analysing CarboxyMethyl Cellulase enzyme activity can reflect the Endoglucanase activity contained in cellulase enzyme. This study aimed to determine the effect of pH and incubation time on the activity of the enzyme cellulase Endoglucanase from Trichoderma reesei and aspergillus niger. The research divided into two phases, each phases was done with 2 variables namely pH and incubation time. pH 4, 5 and 6 for the 1st phase and pH X, pH X.2, pH X.4, pH X.5, pH X.6, and pH X.8 for the 2nd phase. The value of X in the second phase of treatment is the pH value of the first phase which produced the highest Endoglucanase activity. Observation was done for 10 days and were made every 24 hours. Each treatment performed with 3 replicates and treatments carried out with 2 different types of fungus. The pHlevel and incubation time affects the activity of endo-â1,4-glucanase on the enzyme cellulase. The optimum pH condition of cellulose enzyme production with a high endo-â1,4-glucanase produced by Trichoderma reesei is at range of pH 4.5 to pH 6 with an optimum incubation time of 3 to 10 days.While the optimum pH condition of celluloase enzyme production with high endo-â1,4-glucanase produced by Aspergillus niger is at range of pH 5 to pH 5.6 with an optimum incubation time of 8 to 10 days.