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Estimation of Midazolam in Parenterals by RP-HPLC Method

J. V. L. N. Seshagiri Rao and N. Usha Rani

A rapid and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of midazolam in its pure from as well as in parenteral dosage forms. Chromatography was carried out on an ODS column using a mixture of acetonitrile, methanol and water (40 : 20 : 40 v/v) as the mobile phase at a flow rate of 1.0 mL/min. Naphthalene was used as an internal standard and the detection was done at 254 nm. The retention time of the drug was found to be 7.84 min. The method produced linear responses in the concentration range of 0.1 to 16 µg/mL of midazolam. The method was found to be reproducible for analysis of the drug in injections.