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In vitro floral bud synthesis and multiple shoot regeneration studies in watermelon (Citrullus lanatus) from seed explants

R.Chakravarthy, V.N.Koumudi, D.Daniel Kumar, K.Lavanya

A protocol for invitro flowering and multiple shoot regeneration in watermelon (Citrillus lanatus L.) is described. The seeds were excised from mature fruit and cultured on Murashige and Skoog (MS) medium supplemented withBenzyl adenine (BA) 1.0mg/l.After 3 days seedswere germinated, the sprouted seeds were isolated and cultured on MS medium with IndoleBuytric acid (IBA) 0.3mg/l showed efficient rhizogenesis.BA0.75mg/ l +Adenosine (Ads)0.1mg/lt induced proliferated floral buds frominvitro grown shoots, which resulted in synthesis of in vitro flowers, later developed to multiple shoots originating from single node. The rooted shoots were acclimatized effectively in the field.