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Isozymic variation among three filmy ferns belonging to different morphological forms growing in different ecological niche

M.Johnson, S.Dominic Rajkumar, V.Irudayaraj

The present study was aimed to identify isozymic variation among three filmy ferns viz., Trichomones obscurum Bl., Trichomones proliferum Bl. and Trichomones plicatum(V.D.B) Beddomme belonging to differentmorphological forms growing in different ecological niche. The standard technique of vertical poly acrylamide gel electrophoresis was employed to demonstrate the banding profile and the gelswere stained for isoperoxidase enzyme system. A total of sixteen bands were obtained in this enzyme system. Bands were observed in between zone four to nine and other zones were failed to express in this isoperoxidase system. The present biochemical study is in agreement with the abovemorphological and cytological evolution of filmy ferns. Thus the present study clearly shows the genetic distinction of all the three species and supporting the multigeneric systemof classification in filmy ferns.