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L-Glutaminase Production by Aspergillus Wentii Mtcc 1901 under Solid State Fermentation using Mixed Agro Industrial Residues

B. Revanth and K. Jaya Raju

Optimization of physicochemical parameters for production of extracellular L-glutaminase using Aspergillus wentii MTCC 1901 under solid state fermentation is detailed. Substrate screening showed significant results for wheat bran, sesame oil cake (SOC) and green gram husk (GGH). Furthermore, 1 : 1 combination of SOC and GGH showed best results among six different mixed substrate combinations performed. The maximum activity (259.32 U/gd s) of L-glutaminase by Aspergillus wentii MTCC 1901 was obtained using SOC : GGH (1 : 1) of 40% initial moisture content, initial pH 8.0, supplemented with sucrose (4.0% w/v), peptone (2.0% w/v), magnesium sulphate (1% w/v) and L-glutamine (3.0% w/v), inoculated with 2 mL of 96 hours old fungal culture and incubated at 32oC for 96 hours. Both physical and chemical parameters had played a significant role in the production of L-glutaminase. Medium with optimized conditions and supplementation gave a maximum glutaminase activity of 259.32 U/gds, which is above 6 fold increase in activity than that with medium of basal conditions (39.9 U/gds). An antileukemic agent L-glutaminase with relatively good activity is produced from agro industrial residues in a mixed substrate combination.