Abstrato

Melatonin Effect on the Bisphenol-A-Induced Cytotoxicity and Genetic Toxicity in Colon Cancer Cell Line, Normal Gingival Cell Line, and Bone Marrow Stem Cell Line

Mohammad Shokrzadeh, Rouya Ebrahimi, and Nasrin Ghassemi Barghi

The most common path of Human exposure to Bisphenol-A-(BPA) is by oral intake. It involves genotoxicity, oxidative stress, endocrine disruption, mutagenicity, and carcinogenicity in both in vitro and in vivo models. Melatonin has known as a freeradical scavenger and powerful antioxidant agent. This study was initiated to investigate the role of melatonin on viability and genetic disorders of Normal Human Gingival Fibroblasts (HGF), Colon cancer (MKN45), and Bone marrow stem cell lines exposed to BPA. For this purpose, MTT and Comet assays were performed to evaluate the cytotoxicity and genotoxicity properties of BPA and Melatonin role. The results of the present study showed that BPA exposure resulted in increased oxidative stress parameters including MDA, ROS, and decreased GSH content. The current study demonstrated the cytotoxicity and genotoxicity effects of BPA and the protective role of melatonin for preventing cytotoxicity and DNA damage induced by BPA.