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Optimization of high hydrostatic pressure extract 1-deoxynojirimycin from mulberry leaves with response surface methodology

Zhang Pei-Qi, Zhang Li-Hua, Zong Wei

In order to optimum ultra high pressure extraction (UHPE) parameter of 1- deoxynojirimycin (DNJ) from mulberry leaves. On the basis of single-factor test, response surface methodology was used to optimum extraction parameter. The mathematical regression model is established about the dependent variable (extraction yield of DNJ) and independent variables (alcohol concentration, extraction time and extraction pressure) through Box-Benhnken center composite design and response surface methodology. The results indicated that the optimal extraction conditions were alcohol concentration of 73%, extraction time of 3min, extraction pressure of 400 MPa. Under these conditions, the maximum yield of DNJ was 0.0931%. ItÂ’s proved that UHPE was a suitable method for extraction of DNJ as its higher extraction yield and shorter time.