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Quantitative HPLC Analysis of Andrographolide in Andrographis Paniculata at Two Different Stages of Life Cycle of Plant

Meenu Sharma, Aakanksha Sharma And Sandeep

A simple, rapid, selective and quantitative HPLC method has been developed for determination of Andrographolide in Andrographis paniculata at two different stages of life cycle. Whole plant material at two different stages, one after 110 days of plantation i.e., just before flowering (Sample A) and the other at maturity of the crop i.e., bearing flowers, fully matured seed capsules (Sample B) was studied. Mobile phase was methanol: water (65 : 35), flow rate 1.5 mL/min. Retention time of pure Andrographolide was 2.871 minutes. The average andrographolide content varied from 0.81% to 1.86%.