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Screening of some factors affecting bacteriocin production from Lactobacillus curvatus G6 using Plackett-Burman design

Mohamed Sifour, Houria Ouled-Haddar, Tayeb Idoui, Salima Aissaoui, Hiba Namous

Lactobacillus curvatus G6 isolated from newborn feces is capable of producing significant amounts of antimicrobial compounds, especially bacteriocin. Bacteriocin of Lb. curvatus G6 is active against Listeria monocytogenes and methycilin resistant Staphylococcus aureus. The produced bacteriocin showed stability in acidic and neutral pHwith optimal activity at pH 6.0, sensitivity to proteolytic enzyme, and heat stability at different temperatures (from40°C to 100°C). Bacteriocin production started at the beginning of the log phase of the bacterial growth with maximum level after 28 hours (stationary phase). The optimum temperature for the growth (37°C) did correspond with the temperature requirement for the maximumbacteriocin activity. Plackett-Burman design was used to screen factors affecting bacteriocin production by Lb. curvatus G6. Results demonstrated that glucose seems to be the most significant factor affecting bacteriocin production followed by ammonium citrate and peptone.