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Study on factor H mediated identification of staphylococcal Sbi protein in pure culture

Tania Majumdar, Runu Chakraborty, Utpal Raychaudhuri

Staphylococcal Sbi is an IgG binding protein having high affinity for Fc region of human IgG similar to SpA. This bacterial pathogen masks itself with host regulator Factor H and inhibits phagocytosis and opsonization. Factor H has been found to be present in normal human serum (NHS). Thus identification of Factor H facilitates detection of Sbi protein when staphylococcal cells were incubated with NHS. In our present work, we studied the identification of Staphylococcal Sbi protein: Factor H complex using Sodium dodecyl sul fatepolyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analyses with polyclonalanti-Factor H antibodies .